Preferential binding of IFI16 protein to cruciform structure and superhelical DNA

0379230 - BFÚ 2013 RIV US eng J - Článek v odborném periodiku
Brázda, Václav - Coufal, Jan - Liao, J.C.C. - Arrowsmith, Ch.H.
Preferential binding of IFI16 protein to cruciform structure and superhelical DNA.
Biochemical and Biophysical Research Communications. Roč. 422, č. 4 (2012), s. 716-720. ISSN 0006-291X. E-ISSN 1090-2104
Grant CEP: GA ČR(CZ) GAP301/10/1211
Výzkumný záměr: CEZ:AV0Z50040702
Klíčová slova: cruciform structure * IFI16 * protein-DNA binding
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.406, rok: 2012

Interferon (IFN)-inducible HIN-200 proteins play an important role in transcriptional regulation linked to cell cycle control, inflammation, autoimmunity and differentiation. We demonstrate that IFI16 protein binds strongly to negatively superhelical plasmid DNA. We also detect a strong preference for IFI16 binding to cruciform DNA structure compared to linear DNA topology. This novel DNA-binding property of IFI16 protein to scDNA and cruciform structures may play critical roles in its tumor suppressor function.
Trvalý link: http://hdl.handle.net/11104/0210485