0379082 - ÚEB 2013 RIV IE eng J - Článek v odborném periodiku
Cvikrová, Milena - Gemperlová, Lenka - Dobrá, Jana - Martincová, Olga - Prášil, I.T. - Gubiš, J. - Vaňková, Radomíra
Effect of heat stress on polyamine metabolism in proline-over-producing tobacco plants.
Plant Science. Roč. 182, Sp.Issue (2012), s. 49-58. ISSN 0168-9452. E-ISSN 1873-2259
Grant CEP: GA MŠMT OC08013
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Heat stress * Polyamines * Proline
Kód oboru RIV: ED - Fyziologie
Impakt faktor: 2.922, rok: 2012

The effect of heat stress on the accumulation of proline and on the level of polyamines (PAs) in tobacco plants was investigated. Responses to heat stress were compared in the tobacco plants that constitutively over-express a modified gene for the proline biosynthetic enzyme P5CSF129A and in the corresponding wild-type. In the initial phases of heat stress, the accumulation of proline increased in the wild type but slightly decreased in the transformants. The response to heat stress in proline-over-producing tobacco plants involved a transient increase in the levels of free and conjugated putrescine and in the levels of free spermidine, norspermidine and spermine, which correlated with stimulation of the activity of the corresponding biosynthetic enzymes. Diamine oxidase activity increased most significantly in the leaves of WT plants. Polyamine oxidase activity increased in the roots of WT plants and decreased in the leaves and roots of the transformants.
Trvalý link: http://hdl.handle.net/11104/0210356