Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients

0379059 - BTÚ 2013 RIV US eng J - Článek v odborném periodiku
Tomasetti, M. - Staffolani, S. - Nocchi, L. - Neužil, Jiří - Strafella, E. - Manzella, N. - Mariotti, L. - Bracci, M. - Matteo, V.A. - Amati, M. - Santarelli, L.
Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients.
Clinical Biochemistry. Roč. 45, 7-8 (2012), s. 575-581. ISSN 0009-9120. E-ISSN 1873-2933
Grant CEP: GA ČR(CZ) GA204/08/0811
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: Circulating miRNA markers * relative qRT-PCR * pleural mesothelioma
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.450, rok: 2012

Objectives: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. Design and methods: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. Results: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MM patients was strongly associated with worse prognosis. Conclusions: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0210337