Influence of point mutations near the active site on the catalytic properties of fungal arylacetonitrilases from Aspergillus niger and Neurospora crassa

0378903 - MBÚ 2013 RIV NL eng J - Článek v odborném periodiku
Petříčková, Alena - Sosedov, O. - Baum, S. - Stolz, A. - Martínková, Ludmila
Influence of point mutations near the active site on the catalytic properties of fungal arylacetonitrilases from Aspergillus niger and Neurospora crassa.
Journal of Molecular Catalysis B-Enzymatic. Roč. 77, MAY 2012 (2012), s. 74-80. ISSN 1381-1177
Grant CEP: GA ČR(CZ) GAP504/11/0394; GA ČR GD305/09/H008; GA AV ČR IAA500200708; GA MŠMT(CZ) LC06010; GA MŠMT OC09046
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Arylacetonitrilase variants * Aspergillus niger * Neurospora crassa
Kód oboru RIV: CE - Biochemie
Impakt faktor: 2.823, rok: 2012

Arylacetonitrilases from Aspergillus niger CBS 513.88 and Neurospora crassa OR74A (NitAn and NicNc, respectively) were expressed in recombinant Escherichia coil JM109. The respective whole-cell catalysts preferentially hydrolyzed (R)-enantiomers of (R,S)-mandelonitrile and (R,S)-2-phenylpropionitrile. NitNc also formed significant amounts of mandelamide from (R,S)-mandelonitrile (40% of total product), mainly found as the (S)-enantiomer. At pH 4.5 and 5.0, the cells retained more than 40 and 60% of their nitrilase activity at pH 7, respectively
Trvalý link: http://hdl.handle.net/11104/0210223