Antimicrobial Peptide from the Eusocial Bee Halictus sexcinctus Interacting with Model Membranes

0378834 - ÚOCHB 2013 RIV NL eng J - Článek v odborném periodiku
Pazderková, Markéta - Kočišová, E. - Pazderka, T. - Maloň, Petr - Kopecký ml., V. - Monincová, Lenka - Čeřovský, Václav - Bednárová, Lucie
Antimicrobial Peptide from the Eusocial Bee Halictus sexcinctus Interacting with Model Membranes.
Spectroscopy-an International Journal. Roč. 27, 5-6 (2012), s. 497-502. ISSN 0712-4813
Grant CEP: GA ČR GAP208/10/0376
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: antibacterial peptides * halictine * micelle * liposome * phospholipid * circular dichroism * fluorescence * infrared spectroscopy
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 0.530, rok: 2012

Halictine-1 (Hal-1)-a linear antibacterial dodecapeptide isolated from the venom of the eusocial bee Halictus sexcinctus-has been subjected to a detailed spectroscopic study including circular dichroism, fluorescence, and vibrational spectroscopy.We investigated Hal-1 ability to adopt an amphipathic alpha-helical structure upon interaction with model lipid-based bacterial membranes (phosphatidylcholine/phosphatidylglycerol-based large unilamellar vesicles and sodium dodecylsulfate micelles) and helix inducing components (trifluoroethanol). It was found that Hal-1 responds sensitively to the composition of the membrane model and to the peptide/lipid ratio. The amphipathic nature of the helical Hal-1 seems to favour flat charged surfaces of the model lipid particles over the nondirectional interaction with trifluoroethanol. Increasing fraction of polyproline II type conformation was detected at low peptide/lipid ratios.
Trvalý link: http://hdl.handle.net/11104/0210190