From Nucleic Acids Sequences to Molecular Medicine

0378833 - BC 2013 RIV DE eng M - Část monografie knihy
Matoušek, Jaroslav - Riesner, D. - Steger, G.
Viroids: The Smallest Known Infectious Agents Cause Accumulation of Viroid-Specific Small RNAs.
From Nucleic Acids Sequences to Molecular Medicine. Berlin/Heidelberg: Springer Berlin Heidelberg, 2012 - (Erdmann, V.; Barciszewski, J.), s. 629-644. ISBN 978-3-642-27425-1
Grant CEP: GA ČR GCP501/10/J018
Výzkumný záměr: CEZ:AV0Z50510513
Klíčová slova: Pathways of vsRNA production * Potential targets of vsRNA * Transcription factors
Kód oboru RIV: EB - Genetika a molekulární biologie

Viroids are plant-infectious, non-coding, unencapsidated, circular RNAs ranging in size from 250 to 400 nucleotides that are transcribed in a rolling-circle mechanism either in nuclei (Pospiviroidae) or in chloroplasts (Avsunviroidae) of hosts. The pathogenic effect caused by viroids is still an enigma: Potato spindle tuber viroid (PSTVd), the type strain of Pospiviroidae, causes typical symptoms in tomato plants but the severity of symptoms depends on the tomato cultivar; different strains of PSTVd, which vary in sequence by a few mutations from each other, induce symptoms from very mild up to necrosis upon infection of a cultivar. According to recent findings viroids cause the accumulation of viroid-specific small RNAs (vsRNA) similar in size to small interfering (siRNA) and miRNAs, but they do escape the cytoplasmic silencing mechanism. In this review we discuss these findings and hypotheses on the biogenesis of viroid-specific small RNAs and connections to symptom induction.
Trvalý link: http://hdl.handle.net/11104/0218620