Behavior of 4-Hydroxynonenal in Phospholipid Membranes

0378708 - ÚOCHB 2013 RIV US eng J - Článek v odborném periodiku
Vazdar, Mario - Jurkiewicz, Piotr - Hof, Martin - Jungwirth, Pavel - Cwiklik, Lukasz
Behavior of 4-Hydroxynonenal in Phospholipid Membranes.
Journal of Physical Chemistry B. Roč. 116, č. 22 (2012), s. 6411-6415. ISSN 1520-6106. E-ISSN 1520-5207
Grant CEP: GA ČR GA203/08/0114; GA ČR GAP208/10/0376; GA MŠMT LH12001; GA AV ČR GEMEM/09/E006
Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z40400503
Klíčová slova: fluorescence spectroscopy * molecular dynamics * membranes * 4-hydroxynonenal
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.607, rok: 2012

Under conditions of oxidative stress, 4-hydroxy-2-nonenal (4-HNE) is commonly present in vivo. This highly reactive and cytotoxic compound is generated by oxidation of lipids in membranes and can be easily transferred from a membrane to both cytosol and the extracellular space. Employing time-dependent fluorescence shift (TDFS) method and molecular dynamics simulations, we found that 4-HNE is stabilized in the carbonyl region of a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayer. 4-HNE is thus able to react with cell membrane proteins and lipids. Stabilization in the membrane is, however, moderate and a transfer of 4-HNE to either extra- or intracellular space occurs on a microsecond time scale. These molecular-level details of 4-HNE behavior in the lipid membrane rationalize the experimentally observed reactivity of 4-HNE with proteins inside and outside the cell. Furthermore, these results support the view that 4-HNE may play an active role in cell signaling pathways.
Trvalý link: http://hdl.handle.net/11104/0007036