Challenges in analysis of cardiac cell secretomes by using pre-separation by RPLC and tandem mass spectrometry

0378038 - ÚIACH 2013 US eng A - Abstrakt
Šťastná, Miroslava - Goodliffe, L. - Simpson, J. - Van Eyk, J.E.
Challenges in analysis of cardiac cell secretomes by using pre-separation by RPLC and tandem mass spectrometry.
HPLC 2012: 38 th International Symposium on High Performance Liquid Phase Separations and Related Techniques. Anaheim, 2012. P-425-Tue.
[HPLC 2012: International Symposium on High Performance Liquid Phase Separations and Related Techniques /38./. 16.06.2012-21.06.2012, Anaheim]
Institucionální podpora: RVO:68081715
Klíčová slova: secreted proteins * cardiac cells * proteomics * mass spectrometry
Kód oboru RIV: CB - Analytická chemie, separace

We discuss various technical challenges and impediments involved in analysis of proteins secreted by cardiac cells into conditioned media in vitro by using RPLC followed by tandem mass spectrometry.
Trvalý link: http://hdl.handle.net/11104/0210025