Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction

0377953 - ÚOCHB 2013 RIV GB eng J - Článek v odborném periodiku
Ménová, Petra - Hocek, Michal
Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction.
Chemical Communications. Roč. 48, č. 55 (2012), s. 6921-6923. ISSN 1359-7345. E-ISSN 1364-548X
Grant CEP: GA ČR GA203/09/0317
Institucionální podpora: RVO:61388963
Klíčová slova: cross-coupling reactions * nucleoside triphosphates * functionalized DNA * restriction endonucleases * polymerase incorporation
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 6.378, rok: 2012

A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers.
Trvalý link: http://hdl.handle.net/11104/0209962