Proviral integrations of MAV-2 retrovirus in the chicken genome

0377759 - ÚMG 2013 RIV CZ eng E - Elektronický dokument
Pajer, Petr - Pečenka, Vladimír - Karafiát, Vít - Dvořák, Michal
Proviral integrations of MAV-2 retrovirus in the chicken genome.
Praha: Czech Science Foundation, 2012
Grant CEP: GA ČR GA301/09/1727
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: MAV-2 retrovirus * proviral integration sites * insertional mutagenesis
Kód oboru RIV: EB - Genetika a molekulární biologie
http://mav-cis.img.cas.cz/results.html

The table of proviral insertion sites of MAV-2 retrovirus in the chicken genome. Myeloblastosis-associated virus 2 (MAV-2) is a chicken oncogenic retrovirus with the unique ability to induce development of various types of experimental tumors including nephroblastoma, angiosarcoma of lungs, liver and heart, liver carcinoma, cholangiocarcinoma and other rare tumor types. Genes found to be recurrently hit by clonal insertion of the MAV-2 provirus in independent clonal tumors are thought to be cancer genes and their deregulation/mutation is responsible for transformation of the host cell. We used a chicken MAV-2-based model of insertional mutagenesis to identify more than hundred of candidate genes whose deregulation could be responsible for the formation of chicken nephroblastoma, lung angiosarcoma, liver angiosarcoma, hepatocarcinoma and cholangiocarcinoma and for transformation in vitro. Detailed study of the cooperation of MAV-2 insertional mutagenesis and promotional effects of stray cells in lung sarcoma induction lead us to the formulation of the industasis concept. Thus, the single experimental tool, MAV-2 retrovirus, affords the opportunity to study and compare the molecular mechanisms underlying the formation of tumors of different origin and histology as well as contribution of nongenetic factors to tumor formation. The data obtained also allow cross-species comparison of the mechanisms of cell transformation – a necessary step in the effort to understand the complex problem of tumorigenesis.
Trvalý link: http://hdl.handle.net/11104/0216416