High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins

0377502 - MBÚ 2013 RIV NL eng J - Článek v odborném periodiku
Zawadzka-Kazimierczuk, A. - Kozminski, W. - Šanderová, Hana - Krásný, Libor
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins.
Journal of Biomolecular NMR. Roč. 52, č. 4 (2012), s. 329-337. ISSN 0925-2738. E-ISSN 1573-5001
Grant CEP: GA ČR GA204/09/0583
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Intrinsically disordered proteins * Non-uniform sampling * Backbone assignment
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.845, rok: 2012

Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences
Trvalý link: http://hdl.handle.net/11104/0209645