Candida "Psilosis" - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination

0376852 - ÚIACH 2013 RIV GB eng J - Článek v odborném periodiku
Kubesová, Anna - Šalplachta, Jiří - Horká, Marie - Růžička, F. - Šlais, Karel
Candida "Psilosis" - electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination.
Analyst. Roč. 137, č. 8 (2012), s. 1937-1943. ISSN 0003-2654. E-ISSN 1364-5528
Grant CEP: GA AV ČR IAAX00310701
Výzkumný záměr: CEZ:AV0Z40310501
Klíčová slova: Candida parapsilosis * electromigration techniques * MALDI-TOF MS
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 3.969, rok: 2012

Biofilm-positive strains of Candida parapsilosis are the second most common yeasts responsible for bloodstream infections. This pathogen is difficult to identify by standard methods from other phenotypically indistinguishable species, biofilm-negative Candida orthopsilosis and biofilm-positive In this study, the four well-established analytical techniques: gel isoelectric focusing, sodium dodecyl sulfate polyacrylamide gel electrophoresis, two-dimensional gel electrophoresis and matrixassisted laser desorption/ionization time-of-flight mass spectrometry, were applied in order to discriminate C. ‘‘psilosis’’ species.Candida metapsilosis
Trvalý link: http://hdl.handle.net/11104/0209158