Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy

0376810 - FGÚ 2013 RIV GB eng J - Článek v odborném periodiku
Janáček, Jiří - Kreft, M. - Čebašek, V. - Eržen, I.
Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy.
Journal of Microscopy. Roč. 246, č. 2 (2012), s. 107-112. ISSN 0022-2720. E-ISSN 1365-2818
Grant CEP: GA MŠMT(CZ) MEB090910; GA MŠMT(CZ) LC06063
Výzkumný záměr: CEZ:AV0Z50110509
Klíčová slova: capillaries * confocal microscopy * sample deformation * shrinkage * skeletal muscle * 3D
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
Impakt faktor: 1.633, rok: 2012

We studied the deformation in thick sections of the rat skeletal muscle from complete stacks of images captured with confocal microscope. Careful sample preparation and treatment of the tissue cryosections with cold Ringer solution minimize the tissue deformation. Rescaling by the inverse of the axial scaling factor of the stack of optical slices in the direction of the microscope optical axis satisfactorily corrects the axial deformation of skeletal muscle samples
Trvalý link: http://hdl.handle.net/11104/0209120