Počet záznamů: 1  

New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations

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    0376785 - ÚMCH 2013 RIV DE eng J - Článek v odborném periodiku
    Horák, Daniel - Kučerová, J. - Korecká, L. - Jankovičová, B. - Palarčík, J. - Mikulášek, P. - Bílková, Z.
    New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.
    Macromolecular Bioscience. Roč. 12, č. 5 (2012), s. 647-655. ISSN 1616-5187. E-ISSN 1616-5195
    Grant CEP: GA ČR GA203/09/0857; GA ČR GAP503/10/0664; GA MŠMT 7E09109; GA MŠMT 7E12053
    GRANT EU: European Commission(XE) 246513 - NADINE; European Commission(XE) 228980 - CAMINEMS
    Výzkumný záměr: CEZ:AV0Z40500505
    Institucionální podpora: RVO:61389013
    Klíčová slova: enzyme catalysis * glycidyl methacrylate * 2-hydroxyethyl methacrylate
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 3.742, rok: 2012

    Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
    Trvalý link: http://hdl.handle.net/11104/0006971

     
     
Počet záznamů: 1  

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