Počet záznamů: 1
DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions
- 1.0376575 - ÚOCHB 2013 RIV GB eng J - Článek v odborném periodiku
Sorokin, V. A. - Valeev, V. A. - Usenko, E. L. - Andrushchenko, Valery
DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions.
International Journal of Biological Macromolecules. Roč. 50, č. 3 (2012), s. 854-860. ISSN 0141-8130. E-ISSN 1879-0003
Grant CEP: GA ČR GAP208/10/0559
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: DNA metallization * alkaline solution * DNA–metal complex * DNA helix–coil transition * differential UV spectroscopy * thermal denaturation
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 2.596, rok: 2012
Effect of Zn2+ ions on DNA transition from B-form to a metallized form (m-DNA) in Tris and tetraborate buffers at pH 8.5 has been studied by visible and differential UV-spectroscopy and by thermal denaturation. The results have been compared to those obtained at pH 6.5 in cacodylate buffer. It was found that in alkaline solutions Zn2+ ions induced a hypochromicity of the DNA absorption in the whole spectral range monitored, which was attributed to DNA transition from B- to the m-form. Complete metallization occurred only upon heating the DNA solutions containing more than similar to 2 x 10(-4) M of Zn2+ ions. Phase diagrams of the DNA-zinc complexes at pH 6.5 and 8.5 have been obtained for the first time. The m-DNA form showed higher thermal stability compared to B-DNA.
Trvalý link: http://hdl.handle.net/11104/0208939
Počet záznamů: 1