γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis

0375928 - ÚMG 2013 RIV US eng J - Článek v odborném periodiku
Vinopal, Stanislav - Černohorská, Markéta - Sulimenko, Vadym - Sulimenko, Tetyana - Vosecká, Věra - Flemr, Matyáš - Dráberová, Eduarda - Dráber, Pavel
γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis.
PLoS ONE. Roč. 7, č. 1 (2012), e29919. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA MŠMT LC545; GA MŠMT 1M0506; GA ČR(CZ) GD204/09/H084; GA ČR GA204/09/1777; GA AV ČR KAN200520701; GA ČR GAP302/10/1701
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: γ-tubulin 2 * nucleation * embryogenesis
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.730, rok: 2012

While γ-tubulin 1 is ubiquitous, γ-tubulin 2 is known to be not able to substitute for γ-tubulin 1 deficiency in blastocysts. This research article presents our contribution to deciphering of γ-tubulin 2 role. We have found that exogenous γ-tubulin 2 is targeted to centrosomes and interacts with γ-tubulin complex proteins 2 and 4. Depletion of γ-tubulin 1 by RNAi in U2Os cells causes impaired microtubule nucleation and metaphase arrest. Wild-type phenotype in γ-tubulin 1-depleted cells is restored by expression of exogenous mouse and human γ-tubulin 2. Using RT-qPCR and 2D-PAGE, we have found that Tubg2 expression is dramatically reduced in mouse blastocysts. This indicates that γ-tubulin 2 cannot rescue γ-tubulin 1 deficiency in knock-out blastocysts. The obtained data suggest that γ-tubulin 2 is able to nucleate microtubules and subtitute for γ-tubulin 1. We propose that mammalian γ-tubulins are functionally redundant with respect to the nucleation activity of microtubules.
Trvalý link: http://hdl.handle.net/11104/0216424