Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH, and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 genes

0375276 - BC 2012 DE eng C - Konferenční příspěvek (zahraniční konf.)
Matoušek, Jaroslav - Patzak, J. - Kocábek, Tomáš - Füssy, Zoltán - Stehlík, Jan - Orctová, Lidmila - Duraisamy, Ganesh Selvaraj
Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH, and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 genes.
Proceedings of the Scientific Commission, International Hop Growers' Convention I.H.G.C.. Wolnzach: Scientific Commission of the International Hop Growers' Convention, 2011 - (Seigner, E.), s. 54-57. ISSN 1814-2192.
[International Hop Growers' Convention. Lublin (PL), 19.06.2011-23.06.2011]
Grant CEP: GA ČR GA521/08/0740; GA MZe QH81052
Výzkumný záměr: CEZ:AV0Z50510513
Klíčová slova: transcription factor * protein complexes * transient expression assay
Kód oboru RIV: EB - Genetika a molekulární biologie

From a complex lupulin gland-specific cDNA library from Osvald’s 72 hop, we isolated first hop-specific allelic forms of transcription factors (TFs) HlbHLH2 and HlWD40_1, which share high homology with well-described TT8 and TTG1 TFs from A.thaliana, respectively. These TFs are involved in combinatorial control of light-responsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWD40_1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes by interacting with previously isolated HlMyb2 and HlMyb3. The complex formation leads to strong activation of chs_H1 promoter. The activation is dependent on the presence of PMyb-like box CCWAC positioned in 5‘ end of the promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accumulation of valuable lupulin metabolites
Trvalý link: http://hdl.handle.net/11104/0207977