Molecular identification and genotyping of Microsporidia in selected hosts

0375252 - BC 2012 RIV DE eng J - Článek v odborném periodiku
Valenčáková, A. - Balent, P. - Ravaszová, P. - Horák, Aleš - Oborník, Miroslav - Halanová, M. - Malčeková, B. - Novotný, F. - Goldová, M.
Molecular identification and genotyping of Microsporidia in selected hosts.
Parasitology Research. Roč. 110, č. 2 (2012), s. 689-693. ISSN 0932-0113. E-ISSN 1432-1955
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: ENCEPHALITOZOON-CUNICULI * RIBOSOMAL-RNA * SPECIES IDENTIFICATION * AIDS PATIENTS * PET RABBITS
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.852, rok: 2012

The work is described by microscopic, serological (IFAT) and molecular analysis of isolates from clinical samples (blood, faeces and urine) from ten domestic rabbits (Oryctolagus cuniculus), breed MalikA1/2, four New Zealand domestic rabbits, 11 sows of breed Slo0076akian Improved White and 15 clinically healthy laboratory BALB/c mice. The aim of the study was to validate the suitability of species-unspecific primer pairs 530F and 580R for genotype determination of the Microsporidia strain and species-specific primer pairs ECUNF and ECUNR, SINTF and SINTR and EBIER1 and EBIEF1 for the determination of Encephalitozoon cuniculi, E. intestinalis and Enterocytozoon bieneusi species for diagnostic purposes. Sequences of animals were compared with those from the GenBank database. In rabbits, two murine genotypes II and four canine genotypes III were identified. Genotype II was identified in mice. The E. intestinalis identified in the sample from swine showed no genetic heterogeneity.
Trvalý link: http://hdl.handle.net/11104/0207961