Differences in nucleotide excision repair capacity between newly diagnosed colorectal cancer patients and healthy controls

0373763 - ÚEM 2013 RIV GB eng J - Článek v odborném periodiku
Slyšková, Jana - Naccarati, Alessio - Pardini, Barbara - Poláková, Veronika - Vodičková, Ludmila - Šmerhovský, Z. - Levý, M. - Lipská, L. - Liška, V. - Vodička, Pavel (ed.)
Differences in nucleotide excision repair capacity between newly diagnosed colorectal cancer patients and healthy controls.
Mutagenesis. Roč. 27, č. 2 (2012), s. 225-232. ISSN 0267-8357. E-ISSN 1464-3804
Grant CEP: GA ČR GAP304/10/1286; GA MZd NS10230
Grant ostatní: EEA-research fund:(NO) A/CZ0046/2/0012
Výzkumný záměr: CEZ:AV0Z50390512
Klíčová slova: biomarkers * DNA damage * DNA repair capacity
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.500, rok: 2012

DNA damage and nucleotide excision repair capacity (NER-DRC) were assessed in association with sporadic colorectal cancer (CRC) on 70 untreated incident patients and 70 age-matched healthy controls. mRNA expression and polymorphisms in relevant NER genes were concurrently analyzed. Patients had a lower NER-DEC and simultaneously they exhibited higher DNA damage (for both p<0.001) as compared to controls, and these two parameters behaved as independent risk factors. Accumulation of DNA damage and decreasing NER-DRC were reflected by simultaneous increase of CRC risk. Expression levels of 6 out of 9 studied genes differed between groups (p≤0.001), but none of them was related to DRC. Only XPC Ala499Val modulated expression levels of XPC, XPB and XPD gene in patients (p<0.05). This study provides evidence on altered DRC and DNA damage level in sporadic CRC, and highlights the relevance of NER phenotype as a susceptibility biomarker for this malignancy. 1016 Now: 964
Trvalý link: http://hdl.handle.net/11104/0007413