Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94

0372330 - ÚVGZ 2012 RIV US eng J - Článek v odborném periodiku
Prudnikova, T. - Chaloupková, R. - Sato, Y. - Nagata, Y. - Degtjarik, O. - Kutý, Michal - Řezáčová, Pavlína - Damborský, J. - Kutá-Smatanová, Ivana
Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94.
Crystal Growth & Design. Roč. 11, č. 2 (2011), s. 516-519. ISSN 1528-7483. E-ISSN 1528-7505
Grant CEP: GA MŠMT(CZ) LC06010; GA MŠMT(CZ) ED1.1.00/02.0073
Výzkumný záměr: CEZ:AV0Z60870520; CEZ:AV0Z50520514
Klíčová slova: crystal-structure * Japonicum * USDA110 * enzyme
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.720, rok: 2011

The DbeA1 variant of a novel haloalkane dehalogenase DbeA (EC3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA 110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.
Trvalý link: http://hdl.handle.net/11104/0006846