Targeting genes in living mammals by RNA interference

0371760 - ÚMG 2012 RIV GB eng J - Článek v odborném periodiku
Podolská, Kateřina - Svoboda, Petr
Targeting genes in living mammals by RNA interference.
Briefings in Functional Genomics. Roč. 10, č. 4 (2011), s. 238-247. ISSN 2041-2649. E-ISSN 2041-2657
Grant CEP: GA ČR GA204/09/0085; GA ČR GAP305/10/2215; GA MŠMT ME09039
Grant ostatní: EMBO SDIG(XE) 1483
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: RNAi * shRNA * siRNA
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.126, rok: 2011

More than a decade has passed since the discovery of RNA interference (RNAi), an eukaryotic sequence-specific degradation of mRNA induced by complementary double-stranded RNA (dsRNA). RNAi became a common tool for controlled down-regulation of gene expression in cultured cells, as well as in various model organisms. This review summarizes RNAi-based tools for silencing genes in living mammals, which include: (i) transgenic RNAi strategies, where RNAi is triggered by a transgene transmitted through the germline and (ii) approaches, where an RNAi trigger is delivered into an adult animal.
Trvalý link: http://hdl.handle.net/11104/0205204