Native Red Electrophoresis - A new method suitable for separation of native proteins

0371665 - BTÚ 2013 RIV DE eng J - Článek v odborném periodiku
Dráb, T. - Kračmerová, J. - Tichá, I. - Hanzlíková, E. - Tichá, M. - Ryšlavá, H. - Doubnerová, V. - Maňásková-Postlerová, Pavla - Liberda, J.
Native Red Electrophoresis - A new method suitable for separation of native proteins.
Electrophoresis. Roč. 32, č. 24 (2011), s. 3597-3599. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA ČR(CZ) GA303/09/1285
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: native electrophoresis * Ponceau RR * Ponceau S * protein
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.303, rok: 2011

A new type of native electrophoresis was developed to separate and characterize proteins. In this modification of the native blue electrophoresis, the dye Ponceau Red S is used instead of Coomassie Brilliant Blue to impose uniform negative charge on proteins to enable their electrophoretic separation according to their relative molecular masses. As Ponceau Red S binds less tightly to proteins, in comparison with Coomassie Blue, it can be easily removed after the electrophoretic separation and a further investigation of protein properties is made possible (e.g. an enzyme detection or electroblotting). The tested proteins also kept their native properties (enzyme activity or aggregation state).
Trvalý link: http://hdl.handle.net/11104/0205137