Whole-cell oxidation of omeprazole sulfide to enantiopure esomeprazole with Lysinibacillus sp B71

0371609 - MBÚ 2012 RIV NL eng J - Článek v odborném periodiku
Babiak, Petr - Kyslíková, Eva - Štěpánek, Václav - Valešová, Renata - Palyzová, Andrea - Marešová, Helena - Hájíček, J. - Kyslík, Pavel
Whole-cell oxidation of omeprazole sulfide to enantiopure esomeprazole with Lysinibacillus sp B71.
Bioresource Technology. Roč. 102, č. 17 (2011), s. 7621-7626. ISSN 0960-8524. E-ISSN 1873-2976
Grant CEP: GA MŠMT 2B08064
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Biotransformation * Asymmetric oxidation * Esomeprazole
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 4.980, rok: 2011

A Gram-positive bacterium oxidizing omeprazole sulfide to (S)-sulfoxide esomeprazole was isolated from soil polluted with elemental sulfur. The strain exhibited the highest identity with the genus Lysinibacillus and catalyzed oxidation of omeprazole sulfide into enantiopure esomeprazole with conversion of 77% in a stirred bioreactor, fed-batch culture. No consecutive oxidation of (S)-sulfoxide to sulfone was observed during whole-cell catalysis. The unique characteristics of the catalyst provide a solid basis for further improvement and development of sustainable green bioprocess
Trvalý link: http://hdl.handle.net/11104/0205085