Spatial Distribution of Glycerophospholipids in the Ocular Lens

0371571 - MBÚ 2012 RIV US eng J - Článek v odborném periodiku
Pól, Jaroslav - Vidová, Veronika - Hyötyläinen, T. - Volný, Michael - Novák, Petr - Strohalm, Martin - Kostiainen, R. - Havlíček, Vladimír - Wiedmer, S. K. - Holopainen, J.
Spatial Distribution of Glycerophospholipids in the Ocular Lens.
PLoS ONE. Roč. 6, č. 4 (2011), s. 1-9. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA MŠMT LC07017; GA ČR GPP206/10/P018
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: MASS SPECTROMETRY * MEMBRANE-PROTEINS * ALPHA-CRYSTALLIN
Kód oboru RIV: CE - Biochemie
Impakt faktor: 4.092, rok: 2011

Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids
Trvalý link: http://hdl.handle.net/11104/0205056