Effects of 5-azacytidine and trichostatin a on dendritic cell maturation

0371270 - ÚMG 2012 RIV IT eng J - Článek v odborném periodiku
Štěpánek, Ivan - Indrová, Marie - Bieblová, Jana - Fučíková, J. - Spíšek, R. - Bubeník, Jan - Reiniš, Milan
Effects of 5-azacytidine and trichostatin a on dendritic cell maturation.
Journal of Biological Regulators and Homeostatic Agents. Roč. 25, č. 30 (2011), s. 519-531. ISSN 0393-974X. E-ISSN 1724-6083
Grant CEP: GA ČR GAP301/10/2174; GA ČR GA301/09/1024; GA AV ČR IAA500520605
Grant ostatní: EU-FP6(XE) LSHB-CT-2006-018933
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: 5-azacytidine * MHC class I downregulation * tumour chemoimmunotherapy
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.183, rok: 2011

We document the effects of DNA methyltransferase inhibitor 5-azacytidine (5AC) and histone deacetylase inhibitor trichostatin A (TSA) on the murine bone marrow-derived, as well as on the human monocyte-derived dendritic cell (DC) maturation. The major impact of 5AC and TSA on the DC maturation process consisted in the inhibition of unmethylated CpG oligodeoxynucleotide (CpG ODN) 1826 or LPS-induced activation of pro- and anti-inflammatory cytokine gene expression activation. In the in vitro studies, TSA but not 5AC significantly reduced the capacity of the peptide-pulsed DC to induce total spleen as well as CD8+ or CD4+ cell proliferation. IFNγ production by the specific CD4+ spleen cells co-cultured with TSA- but not with 5AC-treated DC was lower, as compared to the cytokine production after co-cultivation with untreated mature DC.
Trvalý link: http://hdl.handle.net/11104/0204822