Fluorescent analysis of boar sperm capacitation process in vitro

0370948 - BTÚ 2012 US eng A - Abstrakt
Děd, Lukáš - Dostálová, Pavla - Dorosh, Andriy - Pěknicová, Jana
Fluorescent analysis of boar sperm capacitation process in vitro.
The book of abstracts - Scientific Program. Portland: Society for the Study of Reproduction, 2011 - (Perreault Darney, S.; Robaire, B.; Murphy, B.). s. 10-10
[44th Annual Meeting Society for the Study of Reproduction : Reproduction and the World's Future. 31.07.2011-04.08.2011, Portland]
Grant CEP: GA MŠMT(CZ) 1M06011
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: capacitation * boar sperm * fluorescent microscopy * flow cytometry * acrosomal reaction
Kód oboru RIV: EI - Biotechnologie a bionika

Capacitation involves physiological changes that spermatozoa must undergo in the female reproductive tract or in vitro to obtain the ability to bind, penetrate and fertilize the egg. The capacitation process was analyzed using four fluorescent techniques (chlortetracycline staining, anti-acrosine antibody assay, anti-phosphotyrosine antibody assay and fluorescein isothiocyanate-conjugated phalloidine assay). All these methods were used in fluorescent microscopy and flow cytometry experiments. According to our results, all selected methods are capable to detect the capacitation progress of boar sperm in vitro, but there are significant differences in this ability in fluorescent microscopy and flow cytometry experimental arrangements as well as in the ability to determine the physiological status of capacitated sperm among the selected methods. Our study thus further contributes to better characterization and understanding of an important step in mammalian reproduction.
Trvalý link: http://hdl.handle.net/11104/0204618