Poly[N-(2-hydroxypropyl)methacrylamide]-Based Tissue-Embedding Medium Compatible with MALDI Mass Spectrometry Imaging Experiments

0370783 - MBÚ 2012 RIV US eng J - Článek v odborném periodiku
Strohalm, Martin - Strohalm, Jiří - Kaftan, Filip - Krásný, Lukáš - Volný, Michael - Novák, Petr - Ulbrich, Karel - Havlíček, Vladimír
Poly[N-(2-hydroxypropyl)methacrylamide]-Based Tissue-Embedding Medium Compatible with MALDI Mass Spectrometry Imaging Experiments.
Analytical Chemistry. Roč. 83, č. 13 (2011), s. 5458-5462. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA MŠMT LC545; GA AV ČR IAAX00500803; GA ČR GPP206/10/P018
Výzkumný záměr: CEZ:AV0Z50200510; CEZ:AV0Z40550506; CEZ:AV0Z40500505
Klíčová slova: SECTIONS * NEUROPEPTIDES * PEPTIDES
Kód oboru RIV: CE - Biochemie
Impakt faktor: 5.856, rok: 2011

Traditional tissue-sectioning techniques for histological samples utilize various embedding media to stabilize the tissue on a sectioning target and to provide a smooth cutting surface. Due to the ion suppression effect in MALDI ionization and number of background peaks in the low-mass region, these media are not suitable for mass spectrometry imaging (MSI) experiments. To overcome this, droplets of water are often used to mount the tissue on a sectioning target, but the ice block formed around the tissue does not provide a good support for sectioning of fragile samples. In this work, we propose a novel embedding media, compatible with MALDI ionization and MSI experiments, based on poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA)
Trvalý link: http://hdl.handle.net/11104/0204477