Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode

0370762 - ÚOCHB 2012 RIV CS eng J - Článek v odborném periodiku
Shetivska, V. - Adam, V. - Prášek, J. - Macek, Tomáš - Macková, M. - Havel, L. - Dioplan, V. - Zehnálek, J. - Hubálek, J. - Kižek, R.
Investigation of the Antioxidant Properties of Metallothionein in Transgenic Tobacco Plants using Voltammetry at a Carbon Paste Electrode.
International Journal of Electrochemical Science. Roč. 6, č. 7 (2011), s. 2869-2883. ISSN 1452-3981. E-ISSN 1452-3981
Grant ostatní: GA ČR(CZ) GA522/07/0692; GA ČR(CZ) GA102/08/1546
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: square wave voltammetry * carbon paste electrode * DNA * metallothionein
Kód oboru RIV: CG - Elektrochemie
Impakt faktor: 3.729, rok: 2011

Electrochemical detection of DNA damage in vitro has been used to evaluate the antioxidant properties of transgenic tobacco plant extracts containing metallothionein (MT). This method is based on assessment of efficiency with which the studied plant extracts are able to reduce to the amount of damage of standard chicken DNA by free OH radicals prepared by the Fenton reaction. We have tested the MT isolated from rabbit liver as a standard reference solution and compared its activity with extracts from wild type tobacco plants, and from twelve clones of transgenic tobacco plants variously carrying human or yeast MT. The present experimental results clearly demonstrate that insertion of a MT gene into the DNA of tobacco plants significantly improves their antioxidant properties in comparison with the non transgenic plants. The method developed for the purpose of this study has been demonstrated to be suitable for rapid screening of various plants with respect to their potential for phytoremediation of heavy metals.
Trvalý link: http://hdl.handle.net/11104/0204462