PIP2: A new key player in the transcription of ribosomal genes - PIP2

0370738 - ÚMG 2012 LT eng A - Abstrakt
Yildirim, Sukriye - Castano, Enrique - Philimonenko, Vlada - Dzijak, Rastislav - Sobol, Margaryta - Hozák, Pavel
PIP2: A new key player in the transcription of ribosomal genes - PIP2.
Program & Abstracts, 22nd Wilhelm Bernhard Workshop. Riga: Latvian Institute of Organic Synthesis, 2011. s. 21-21.
[Wilhelm Berhnard Workshop /22./. 22.08.2011-25.08.2011, Riga]
Grant CEP: GA ČR GAP305/11/2232; GA MŠMT LC545; GA MŠMT(CZ) LC06063; GA ČR(CZ) GD204/09/H084
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: PIP2 * phospholipids * nucleus * transcription
Kód oboru RIV: EB - Genetika a molekulární biologie

Phosphatidylinositol-4,5-bisphosphate (PIP2) resides in the nucleus in a different form than the classical bilayer membrane, apparently forming specific nuclear protein complexes. There is limited data on the function of PIP2 in the nucleus. Yet, it was shown that PIP2 binding to histones reverses the inhibitory effect of histones on transcription in Drosophila (Yu et al., 1998). In this study we focused in the function of PIP2 in ribosomal gene transcription and showed that PIP2 binds to transcription machinery. Removal of PIP2 from in vitro transcription assays caused the inhibiton of transcription for ribosomal genes and it was possible to recover the transcription efficiency by adding back PIP2 to the transcription reaction. The data suggest that nucleolar PIP2 might serve as a transcription factor for ribosomal genes.
Trvalý link: http://hdl.handle.net/11104/0204447