An activated immune and inflammatory response targets the pancreas of newborn pigs with cystic fibrosis

0370690 - MBÚ 2012 RIV CH eng J - Článek v odborném periodiku
Abu-El-Haija, M. - Šinkora, Marek - Meyerholz, D. K. - Welsh, M. J. - McCray, P.B. - Butler, J. E. - Uc, A.
An activated immune and inflammatory response targets the pancreas of newborn pigs with cystic fibrosis.
Pancreatology. Roč. 11, č. 5 (2011), s. 506-515. ISSN 1424-3903. E-ISSN 1424-3911
Grant CEP: GA MŠMT ME09089
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: cystic fibrosis * pancreatitis * flow cytometry
Kód oboru RIV: EC - Imunologie
Impakt faktor: 1.987, rok: 2011

In cystic fibrosis (CF), pancreatic disease begins in utero and progresses over time to complete destruction of the organ. Although inflammatory cells have been detected in the pancreas of humans and pigs with CF, their subtypes have not been characterized. Using four-color flow cytometry, we analyzed the surface antigens of leukocytes in pancreas, blood, and mesenteric lymph nodes (MLN) of newborn pigs with CF (CFTR –/– and CFTRdF508/dF508) and in those without CF (CFTR+ /– , CFTR +/dF508 , CFTR+ /+). Pancreatic histopathology was examined with HE stain. CF pig pancreas had patchy distribution of inflammatory cells with neutrophils / macrophages in dilated acini, and lymphocytes in the interstitium compared to non-CF
Trvalý link: http://hdl.handle.net/11104/0204413