Real time in vivo monitoring of cytotoxic activity of two different antimicrobial peptides lasioglossin III and lasiocepsin

0370641 - ÚOCHB 2012 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Tůmová, Tereza - Lovecká, P. - Čeřovský, Václav - Slaninová, Jiřina
Real time in vivo monitoring of cytotoxic activity of two different antimicrobial peptides lasioglossin III and lasiocepsin.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 151-153. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant CEP: GA ČR GA203/08/0536
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: Lasioglossum laticeps * cytotoxic activity * real time assay * impedance based assay
Kód oboru RIV: CC - Organická chemie

Real-time cell analyzer dual-plate (RTCA DP) can be used to dynamically test the cytotoxic activity of potent antimicrobial peptides. In this study, we tested the activity of two different antimicrobial peptides isolated from venom reservoirs of a wild bee against normal mammalian cell line – rat intestinal epithelial cells. The cytotoxic effect monitored by RTCA DP was expressed as IC50 and compared with the results of standard cytotoxic tests.
Trvalý link: http://hdl.handle.net/11104/0204376