Larvae of flesh fly Neobellieria bullata as a source for novel antimicrobial peptides

0370593 - ÚOCHB 2012 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Neubauerová, Tereza - Macková, Martina - Macek, Tomáš - Šanda, Miloslav - Voburka, Zdeněk
Larvae of flesh fly Neobellieria bullata as a source for novel antimicrobial peptides.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 100-102. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant ostatní: GA ČR(CZ) GA522/09/1693; GA ČR(CZ) GD305/09/H008
Program: GA; GD
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: peptide synthesis * Neobellieria bullata * antimicrobial peptide
Kód oboru RIV: CC - Organická chemie

The antibiotic resistant strains of microorganisms are increasing threat for public health. Antimicrobial peptides from natural sources display a wide spectrum of activity. In our laboratory, several peptide fractions with antimicrobial acivity against gram-positive and gramnegative bacteria and fungi were isolated from larvae of flesh fly Neobellieria bullata. The presence of peptides was proved by tricine electrophoresis, molecular weights and sequences determination by MS and Edman sequencing, respectively. In comparison with database sequences we obtained structures present mainly in insect and amphibian antimicrobial peptides, but total matches were not higher than in 36%. Upon the obtained sequence motifs we designed structures of several synthetic peptides. These were synthetised on the Rink amide MBHA resin. Synthesis was performed according Fmoc/PyBOP-DIEA protocol. Antimicrobial activity of these peptides was determined.
Trvalý link: http://hdl.handle.net/11104/0204344