Anovel defensin from the mucus of the wood wasp Xiphydria camelus

0370590 - ÚOCHB 2012 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Monincová, Lenka - Fučík, Vladimír - Voburka, Zdeněk - Cvačka, Josef - Čeřovský, Václav - Šrůtka, P.
Anovel defensin from the mucus of the wood wasp Xiphydria camelus.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 86-89. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant CEP: GA ČR GA203/08/0536
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: insect defensin * Xiphydria camelus * antimicrobial activity * mass spectrometry * sequencing
Kód oboru RIV: CC - Organická chemie

We have isolated a novel defensin from the mucus of the wood wasp Xiphydria camelus. The Edman degradation in 50 cycles revealed a peptide sequence similar to other insect defensins. The complete 55 amino acid residues sequence of X. camelus defensin was proposed based on the mass spectrometry analysis and by the deduction based on the comparison with the sequences of other hymenopteran defensins. The defensin was tested against both Gram-positive and -negative bacteria.
Trvalý link: http://hdl.handle.net/11104/0204341