Design and synthesis of specific colevelin derivatives for in vitro and in vivo studies

0370587 - ÚOCHB 2012 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Kostomoiri, M. - Zikos, C. - Benaki, D. - Slaninová, Jiřina - Pirmettis, I. - Papadopoulos, M. - Pelecanou, M. - Livaniou, E.
Design and synthesis of specific colevelin derivatives for in vitro and in vivo studies.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 70-72. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: neuroprotective peptides * colivelin * humanin * Alzheimer's disease
Kód oboru RIV: CC - Organická chemie

Colivelin is the most potent member of the humanin family of neuroprotective peptides with in vitro and in vivo action against insults associated with Alzeimer’s Disease. The mechanism of action of colivelin is not yet elucidated. The objective of the present study is the design and synthesis of specific colivelin derivatives that can be used as molecular probes in in vitro and in vivo biological experiments. In the frame of the study three colivelin derivatives bearing suitable tags, i.e the fluorescent moiety FITC, the 99mTc-radiometal chelating unit dimethylGly-Ser-Cys, and the streptavidin-counterpart biotinyl-group, were designed and synthesized. These derivatives will be used in in vitro cell component binding and in vivo biodistribution experiments, in order to gain better insight on the mode of the neuroprotective action of colivelin.
Trvalý link: http://hdl.handle.net/11104/0204339