Analysis of antimicrobial peptides by capillary electrophoresis

0370490 - ÚOCHB 2012 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Ehala, Sille - Niederhafner, Petr - Čeřovský, Václav - Řezanka, P. - Sýkora, D. - Král, V. - Kašička, Václav
Analysis of antimicrobial peptides by capillary electrophoresis.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 37-40. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant CEP: GA ČR(CZ) GA203/09/0675; GA ČR(CZ) GA203/08/1428
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: capillary electrophoresis * antimicrobial peptides * gold nanoparticles
Kód oboru RIV: CC - Organická chemie

Capillary electrophoresis was used for the purity degree determination of new antimicrobial peptides: octadecapeptide melectin and tetradecapeptide mastoparan PDD-B, and their synthetic analogues. Besides, four semiempirical models correlating electrophoretic mobilities of these peptides with their charge and size (relative molecular mass) were applied to predict their probable structures in solution. Additionally, capillaries coated with gold nanoparticles were employed for the separation of mixtures of antimicrobial peptides.
Trvalý link: http://hdl.handle.net/11104/0204285