Monitoring of peptide induced disruption of artificial lipid membrane constructed on boron-doped nanocrystalline diamond by electrochemical impedance spectroscopy

0370340 - ÚOCHB 2012 RIV DE eng J - Článek v odborném periodiku
Petrák, V. - Grieten, L. - Taylor, Andrew - Fendrych, František - Ledvina, Miroslav - Janssens, S. D. - Nesládek, M. - Haenen, K. - Wagner, P.
Monitoring of peptide induced disruption of artificial lipid membrane constructed on boron-doped nanocrystalline diamond by electrochemical impedance spectroscopy.
Physica Status Solidi A. Roč. 208, č. 9 (2011), s. 2099-2103. ISSN 1862-6300. E-ISSN 1862-6319
Grant CEP: GA AV ČR KAN200100801; GA AV ČR(CZ) KAN400480701; GA MŠMT(CZ) LD11076
GRANT EU: European Commission(XE) 238201 - MATCON
Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z10100520
Klíčová slova: biosensor * boron-doped nanocrystalline diamond * electrochemical impedance spectroscopy
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 1.463, rok: 2011

With the rise of antibiotic resistance of pathogenic bacteria there is an increased demand for monitoring of the functionality of bacteria membranes, whose disruption can be induced by peptide lipid interactions. In this work we attempt to monitor formation and subsequent peptide induced disruption of supported lipid membranes (SLBs) on boron-doped nanocrystalline diamond (B-NCD). Electrochemical Impedance Spectroscopy (EIS) was used to study in situ changes related to lipid membrane formation and disruption by peptide-induced interactions. The observed impedance changes were minimal for oxidized B-NCD samples. The sensitivity for the detection of membrane formation and disruption was significantly higher for hydrogenated B-NCD surfaces. Data modelling indicates large differences in the structure of electrical double layer at the B-NCD/SLB interface for hydrogen and oxygen terminated B-NCD surfaces. For oxidized B-NCD surfaces, EIS changes are negligible.
Trvalý link: http://hdl.handle.net/11104/0204175