Redox labelling of nucleic acids for analyzing nucleotide sequences and monitoring DNA-protein interactions

0361621 - ÚOCHB 2012 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Fojta, Miroslav - Havran, Luděk - Horáková Brázdilová, Petra - Pivoňková, Hana - Kostečka, Pavel - Macíčková-Cahová, Hana - Raindlová, Veronika - Vrábel, Milan - Hocek, Michal
Redox labelling of nucleic acids for analyzing nucleotide sequences and monitoring DNA-protein interactions.
Chemistry of Nucleic Acid Components. 15th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i., 2011 - (Hocek, M.), s. 155-158. Collection Symposium Series, 12. ISBN 978-80-86241-37-1.
[Chemistry of Nucleic Acid Components /15./. Český Krumlov (CZ), 05.06.2011-10.06.2011]
Grant CEP: GA MŠMT LC512; GA MŠMT(CZ) LC06035; GA AV ČR(CZ) IAA400040901; GA AV ČR(CZ) IAA400040903; GA ČR GA203/09/0317; GA AV ČR(CZ) GPP206/11/P739
Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z50040507; CEZ:AV0Z50040702
Klíčová slova: DNA * electrochemistry * redox labeling
Kód oboru RIV: CC - Organická chemie

Nucleobase labelling of DNA for electrochemical sensing was attained through chemical modification of thymine bases with osmium tetroxide in the presence of nitrogenous ligands, or via enzymatic incorporation of nucleotide conjugates with redox-active moieties using labelled deoxynucleoside triphosphates. DNA hybridization, primer extension and PCR techniques were used for sequence-specific DNA assays. Tail-labelled DNA substrates were applied to monitor DNA binding by tumour suppressor p53 protein.
Trvalý link: http://hdl.handle.net/11104/0198897