Identification of morphological similar species of genus monilinia by capillary and gel format of the electromigration techniques and MALDI-TOF MS

0361141 - ÚIACH 2012 HU eng A - Abstrakt
Kubesová, Anna - Horká, Marie - Šalplachta, Jiří - Zapletalová, E. - Horký, J.
Identification of morphological similar species of genus monilinia by capillary and gel format of the electromigration techniques and MALDI-TOF MS.
HPLC 2011. 36 th International Symposium on High-Performance Liquid Phase Separations and Related Techniques. 2011. P1-G-226-TU. ISBN 978-963-89335-0-8.
[International Symposium on High-Performance Liquid Phase Separations and Related Techniques /36./. 19.06.2011-23.06.2011, Budapest]
Grant CEP: GA AV ČR IAAX00310701
Výzkumný záměr: CEZ:AV0Z40310501
Klíčová slova: CIEF * CZE * gel IEF
Kód oboru RIV: CB - Analytická chemie, separace

This research will build on our experimental knowledge arising from the CIEF and CZE separation of the hydrophobic conidia from the cultures of different strains of the filamentous fungi, Aspergillus, Fusarium and Penicillium. Now we develop rapid and reproducible methods for identification of intact spores of Monilinia spp. based on off-line combination CZE, CIEF, gel IEF, SDS-PAGE and MALDI-TOF MS techniques. The optimized methods can be applied also at the identification of the Monilinia spp. from the real plant samples and at the identification of four M. polystroma strains. The results were compared with those obtained by conventional microbiological identification procedures.
Trvalý link: http://hdl.handle.net/11104/0198535