Počet záznamů: 1
c-Myb expression at different stages of molar tooth germ osseointegration
- 1.0359668 - ÚŽFG 2012 GB eng A - Abstrakt
Matalová, Eva - Radlanski, R. - Míšek, Ivan - Šmarda, J.
c-Myb expression at different stages of molar tooth germ osseointegration.
Bone. Elsevier. Roč. 44, Suppl. 1 (2009), S161-S161. ISSN 8756-3282. E-ISSN 1873-2763.
[Joint Meeting of the International-Bone-and-Mineral-Society /2./. 21.05.2009-25.05.2009, Sydney]
Výzkumný záměr: CEZ:AV0Z50450515
Klíčová slova: c-Myb
Kód oboru RIV: FB - Endokrinologie, diabetologie, metabolizmus, výživa
The aim was to show c-Myb protein expression profile in temporo-spatial model of mouse molar tooth germ development in the context of related tissues. Expression of c-Myb (Abcam polyclonal antibody) was followed together with proliferation analysis (PCNA –proliferating cell nuclear antigen, DAKO monoclonal antibody) and apoptosis detection (TUNEL test, terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, Chemicon). Secondary biotinylated antibody was labelled by streptavidin-POD to visualize positive cells by addition of DAB substrate. c-Myb, PCNA and TUNEL were evaluated in the epithelial and mesenchymal parts of the tooth germ and in the surrounding interdental bone.
Trvalý link: http://hdl.handle.net/11104/0197412
Počet záznamů: 1