Toxicity of complex cyanobacterial samples and their fractions in Xenopus laevis embryos and the role of microcystins

0358743 - BÚ 2012 RIV NL eng J - Článek v odborném periodiku
Buryšková, B. - Hilscherová, Klára - Babica, Pavel - Vršková, D. - Maršálek, Blahoslav - Bláha, Luděk
Toxicity of complex cyanobacterial samples and their fractions in Xenopus laevis embryos and the role of microcystins.
Aquatic Toxicology. Roč. 80, č. 4 (2006), s. 346-354. ISSN 0166-445X. E-ISSN 1879-1514
Grant CEP: GA MŠMT 1M0571; GA AV ČR KJB6005411
Výzkumný záměr: CEZ:AV0Z60050516
Klíčová slova: FETAX * Xenopus laevis * malformations * cyanobacterial fractions * biomarkers
Kód oboru RIV: EF - Botanika
Impakt faktor: 2.964, rok: 2006

The object of this work was to evaluate the effects of various cyanobacterial fractions in Frog Embryo Teratogenesis Assay Xenopus (FETAX) with African clawed frog embryos. Fractions were prepared from five biomasses with different dominant genera (Microcystis, Aphanizomenon, Anabaena, Planktothrix) and different microcystin content. Effects of following fractions were investigated: (I) homogenate of complex cyanobacterial biomass, (II) cell debris (pellet) after centrifugation of complex biomass, (III) supernatant after centrifugation of complex biomass (= crude aqueous extract), (IV) permeate after passing of crude extract through C-18 column (fraction devoid of microcystins), (V) eluate from C-18 column (containing microcysins, if present).
Trvalý link: http://hdl.handle.net/11104/0196689