Identification of Toll-like receptor genes in Grey Partridge (Perdix perdix)

0357769 - ÚBO 2011 CZ eng A - Abstrakt
Tomášek, Oldřich - Vinkler, Michal - Bainová, H. - Opatová, Pavlína - Bryjová, Anna - Svobodová, J. - Albrecht, Tomáš
Identification of Toll-like receptor genes in Grey Partridge (Perdix perdix).
Zoologické dny Brno 2011: sborník abstraktů z konference 17.-18. února 2011. Brno: Ústav biologie obratlovců AVČR, 2011 - (Bryja, J.; Řehák, Z.; Zukal, J.). s. 231-232. ISBN 978-80-87189-09-2.
[Zoologické dny. 17.02.2011-18.02.2011, Brno]
Výzkumný záměr: CEZ:AV0Z60930519
Klíčová slova: Grey Partridge * Toll-like receptor
Kód oboru RIV: EG - Zoologie
http://zoo.ivb.cz/doc/sborniky/sbornik_2011.pdf

In the last decade, after recognising their important role in immunity, increasing attention has been paid to genes coding Toll-like receptors (TLRs). These membrane-bound receptors detect conserved molecular patterns of pathogenic microorganisms (pathogen associated molecular patterns; PAMPs), e.g. lipopolysaccharide (LPS), flagellin or viral RNA. Thus, TLRs serve as a crucial component of the detection of infections. Activated TLRs trigger the effector innate immunity mechanisms and provide „signal of danger“ necessary for the activation of lymphocytes, representing the bridge between innate and adaptive immunity. Most of the knowledge of avian TLRs has been obtained in chicken (Gallus gallus). Although some of the receptors were described also in duck (Anas platyrhynchos), turkey (Meleagris gallopavo) and zebra finch (Teaniopygia guttata), no systematic research on TLR repertoire has been made in any free living bird species. Here we report the identification of orthologues of chicken TLRs (chTLRs) genes in Grey Partridge (Perdix perdix). Using primers based on known chTLRs sequences, we have identified orthologues of chTlr1LA, chTlr2A, chTlr3, chTlrR4, chTlr5, chTlr7, chTlr15 and chTlr21. All sequences of Grey Partridge TLRs (ppTLRs) genes showed high similarity with their chicken orthologues and yielded meaningful polypeptide sequences. Analysis of these amino acid sequences by SMART revealed a structure similar to their chicken orthologues including extracellular region with leucin rich repeats (LRRs), transmembrane domain and intracytoplasmic Toll/interleukin-1 receptor (TIR) domain. Phylogenetic analysis confirmed the close proximity of ppTlrs to chTlrs, and both together clustered with predicted sequences of orthologue Tlrs of zebra finch obtained from molecular sequence databases.
Trvalý link: http://hdl.handle.net/11104/0195966