Compact multi-channel surface plasmon resonance sensor for rapid detection of bacterial pathogens

0356070 - ÚFE 2011 CZ eng A - Abstrakt
Vala, Milan - Šípová, Hana - Špringer, Tomáš - Chadt, Karel - Piliarik, Marek - Homola, Jiří
Compact multi-channel surface plasmon resonance sensor for rapid detection of bacterial pathogens.
EUROPT(R)ODE X. Vol. XConference on Optical Chemical Sensors and Biosensors. Praha: Institute of Photonics and Electronics AS CR, v.v.i, 2010 - (Homola, J.). s. 214-214. ISBN 978-80-86269-20-7.
[EUROPT(R)ODE X – X.Conference on Optical Chemical Sensors and Biosensors. 28.03.2010-31.3.2010, Praha]
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: surface plasmon resonance * biosensor * detection of pathogens
Kód oboru RIV: JB - Senzory, čidla, měření a regulace

We report a novel compact surface plasmon resonance (SPR) sensor capable of simultaneous detection of up to 10 analytes. The sensor is based on angular interrogation of the SPR on a diffraction grating. Prototype of the sensor was characterized in a refractometric experiment and its resolution was 6x10 7 RIU. Limit of detection of short oligonucleotides was below 1nM concentration. Sensor also detected milk samples spiked with 103 cfu/ml and 104 cfu/ml of E. coli and S. Aureus, respectively.
Trvalý link: http://hdl.handle.net/11104/0194690