Real-time monitoring of biomolecular interactions in blood plasma using surface plasmon resonance

0356069 - ÚFE 2011 CZ eng A - Abstrakt
Špringer, Tomáš - Piliarik, Marek - Homola, Jiří
Real-time monitoring of biomolecular interactions in blood plasma using surface plasmon resonance.
EUROPT(R)ODE X. Vol. XConference on Optical Chemical Sensors and Biosensors. Praha: Institute of Photonics and Electronics AS CR, v.v.i, 2010 - (Homola, J.). s. 226-226. ISBN 978-80-86269-20-7.
[EUROPT(R)ODE X – X.Conference on Optical Chemical Sensors and Biosensors. 28.03.2010-31.3.2010, Praha]
Grant CEP: GA AV ČR KAN200670701
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: surface plasmon resonance * molecular interaction analysis * microfluidics * optical biosensor
Kód oboru RIV: JB - Senzory, čidla, měření a regulace

We report a novel approach to biomolecular interaction analysis which allows real-time monitoring of interactions in complex media. The investi¬gated complex sample (e.g. blood plasma) and a washing solution are al¬ternately injected in the flow-chamber of a surface plasmon resonance biosensor. Immediate switching between these liquids and removal of non-specifi¬cally adsorbed material on a chip surface allows assessing the actual level of bound analyte in the course of the binding.
Trvalý link: http://hdl.handle.net/11104/0194689