Streptavidin-enhanced sandwich assay for specific detection of single nucleotide polymorphism

0356067 - ÚFE 2011 CZ eng A - Abstrakt
Šípová, Hana - Špringer, Tomáš - Homola, Jiří
Streptavidin-enhanced sandwich assay for specific detection of single nucleotide polymorphism.
EUROPT(R)ODE X. Vol. XConference on Optical Chemical Sensors and Biosensors. Praha: Institute of Photonics and Electronics AS CR, v.v.i, 2010 - (Homola, J.). s. 220-220. ISBN 978-80-86269-20-7.
[EUROPT(R)ODE X – X.Conference on Optical Chemical Sensors and Biosensors. 28.03.2010-31.3.2010, Praha]
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: Surface Plasmon Resonance * single nucleotide polymorphism * amplification
Kód oboru RIV: JB - Senzory, čidla, měření a regulace

This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance (SPR) biosensor technology. In this assay, a part of the target DNA is recognized by a probe coupled with streptavidin-oligonucleotide (SON) complexes ex-situ. The SON-target complex is then detected with the SPR sensor with high specificity and sensitivity. The assay is applied to detection of short analogue of TP53.
Trvalý link: http://hdl.handle.net/11104/0194687