Protein markers of myelodysplastic syndrome for SPR chip analysis

0356065 - ÚFE 2011 CZ eng A - Abstrakt
Pimková, K. - Hegnerová, Kateřina - Májek, P. - Kotlín, R. - Suttnar, J. - Homola, Jiří - Dyr, J. E.
Protein markers of myelodysplastic syndrome for SPR chip analysis.
EUROPT(R)ODE X. Vol. XConference on Optical Chemical Sensors and Biosensors. Praha: Institute of Photonics and Electronics AS CR, v.v.i, 2010 - (Homola, J.). s. 230-230. ISBN 978-80-86269-20-7.
[EUROPT(R)ODE X – X.Conference on Optical Chemical Sensors and Biosensors. 28.03.2010-31.3.2010, Praha]
Grant CEP: GA AV ČR KAN200670701
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: vascular endothelial growth receptor 1 (VEGF) * surface plasmon resonance sensor * myelodysplastic syndrom
Kód oboru RIV: JB - Senzory, čidla, měření a regulace

The myelodysplastic syndromes are clonal hematologic disorders characterized clinically and morphologically by ineffective hematopoiesis with a high risk of progression to acute myeloid leukemia (AML). Mutual protein-protein interaction was measured by a direct, real-time and label free technique measuring refractive index changes produced by the binding of analyte to its biospecific partner immobilized on the sensor surface. Our task was to optimise conditions for interaction of each pair of proteins, especially find out optimal concentration, minimal detection limit and suitable measurement buffer.
Trvalý link: http://hdl.handle.net/11104/0194685