Human plasma depletion techniques for label-free detection of low-abundance plasma proteins

0356063 - ÚFE 2011 CZ eng A - Abstrakt
Suttnar, J. - Bocková, Markéta - Pimková, K. - Májek, P. - Kotlín, R. - Homola, Jiří - Dyr, J. E.
Human plasma depletion techniques for label-free detection of low-abundance plasma proteins.
EUROPT(R)ODE X. Vol. XConference on Optical Chemical Sensors and Biosensors. Praha: Institute of Photonics and Electronics AS CR, v.v.i, 2010 - (Homola, J.). s. 238-238. ISBN 978-80-86269-20-7.
[EUROPT(R)ODE X – X.Conference on Optical Chemical Sensors and Biosensors. 28.03.2010-31.3.2010, Praha]
Grant CEP: GA AV ČR KAN200670701
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: plasma imunodepletion * peptide library * surface plasmon resonance
Kód oboru RIV: JB - Senzory, čidla, měření a regulace

This paper describes the influence of blood plasma depletion on the performance of surface plasmon resonance sensor’s (SPR) estimation of proteins. Depletion method utilizing immobilized combinatorial hexapeptide ligand library or restricted acces material were used for samples pretreatment. The nonspecific binding of depleted plasma on the sensor surface was half as much lower than the binding of whole plasma with the same concentration of low-abundant proteins.
Trvalý link: http://hdl.handle.net/11104/0194683