Analysis of the N-terminus of nucleocapsid protein of Mason-Pfizer monkey virus: its role in the particle assembly

0355949 - ÚOCHB 2011 IT eng C - Konferenční příspěvek (zahraniční konf.)
Böhmová, Karolína - Hadravová, Romana - Štokrová, Jitka - Ruml, T. - Pichová, Iva - Rumlová, Michaela
Analysis of the N-terminus of nucleocapsid protein of Mason-Pfizer monkey virus: its role in the particle assembly.
Centennial Retrovirus Meeting. Bologna: Medimond, 2010 - (Daniel, R.; Hejnar, J.; Skalka, A.; Svoboda, J.), s. 13-17
[Centennial Retrovirus Meeting. Praha (CZ), 29.04.2010-04.05.2010]
Grant ostatní: GA MŠk(CZ) ME 904
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: Mason-Pfizer monkey virus * basic region * immature-like particles
Kód oboru RIV: CE - Biochemie

Assembly of immature retroviral particles is mediated mainly by mutual interactions of capsid (CA) and nucleocapsid (NC) domains of Gag polyproteins and viral genomic RNA. We identified a “basic domain” (NC16-23) at the N-terminus of Mason-Pfizer monkey virus (M-PMV) NC that contributes to the oligomerization of CA leading to the assembly of virus-like particles (VLP). Within this domain we identified a stretch of the three lysine residues K16, K18, and K20 crucial for the assembly. The basic region can be substituted by non-specific positively charged peptide. In summary, we report here the importance of basic residues at the N-terminus of M-PMV NC for the assembly of immature-like particles.
Trvalý link: http://hdl.handle.net/11104/0194598