Organization, regulation and expression of nitrile degradation genes of Rhodococcus erythropolis

0354660 - MBÚ 2011 ES eng C - Konferenční příspěvek (zahraniční konf.)
Pátek, Miroslav - Knoppová, Monika - Volkova, O. - Pavlík, A. - Kubáč, David - Nešvera, Jan - Martínková, Ludmila
Organization, regulation and expression of nitrile degradation genes of Rhodococcus erythropolis.
New Biotechnology. Barcelona: European Federation of Biotechnology, 2016, s. 104-104. ISSN 1871-6784. E-ISSN 1876-4347.
[European Congress on Biotechnology /14./. Barcelona (ES), 13.09.2009-16.09.2009]
Grant CEP: GA MŠMT(CZ) LC06010; GA MŠMT OC09046
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: nucleotide sequence
Kód oboru RIV: EE - Mikrobiologie, virologie

The complete nucleotide sequence of the cluster (9552 bp, GenBank Acc. No. AM946017) also covered the genes nhr1, nhr2, nhr3 and nhr4 coding for regulatory proteins. All genes of the cluster are transcribed in the same direction. The regions upstream of each gene, potentially containing promoters, were cloned in the promoter-probe vector pEPR1 and their activity in R. erythropolis was analyzed using fluorescence of the GFP (green florescent protein) reporter. Promoter of the ami gene was localized by determination of the ami transcription start point using primer extension analysis
Trvalý link: http://hdl.handle.net/11104/0193616