The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito, Ochlerotatus triseriatus

0351986 - BC 2011 US eng J - Článek v odborném periodiku
Calvo, E. - Sanchez-Vargas, I. - Kotsyfakis, Michalis - Favreau, A.J. - Barbian, K.D. - Pham, V.M. - Olson, K.E. - Ribeiro, J.M.C.
The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito, Ochlerotatus triseriatus.
Journal of Medical Entomology. Roč. 47, č. 3 (2010), s. 376-386. ISSN 0022-2585. E-ISSN 1938-2928
Klíčová slova: mosquito * salivary gland * sialome * transcriptome * La Crosse virus
Impakt faktor: 1.925, rok: 2010

Saliva of blood-sucking arthropods contains a complex mixture of peptides that affect their host's hemostasis, inflammation, and immunity. These activities can also modify the site of pathogen delivery and increase disease transmission. The mosquito Ochlerotatus triseriatus is a vector of La Crosse virus and produces allergy in humans. In this work, a total of 1,575 clones randomly selected from an adult female O. triseriatus salivary gland cDNA library was sequenced and used to assemble a database that yielded 731 clusters of related sequences, 560 of which were singletons. Primer extension experiments were performed in selected clones to further extend sequence coverage, allowing for the identification of 159 protein sequences, 66 of which code for putative secreted proteins.
Trvalý link: http://hdl.handle.net/11104/0191603