0350621 - ÚFE 2011 RIV GB eng J - Článek v odborném periodiku
Šípová, Hana - Vaisocherová, Hana - Štepánek, J. - Homola, Jiří
A dual surface plasmon resonance assay for the determination of ribonuclease H activity.
Biosensors and Bioelectronics. Roč. 26, č. 4 (2010), s. 1605-1611. ISSN 0956-5663. E-ISSN 1873-4235
Grant CEP: GA AV ČR KAN200670701; GA MŠMT OC09058; GA ČR GA202/09/0193
Grant ostatní: Univerzita Karlova(CZ) SVV-2010-261 304
Výzkumný záměr: CEZ:AV0Z20670512
Klíčová slova: Surface plasmon resonance * Enzyme activity assay * Ribonuclease H * Biosensor
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
Impakt faktor: 5.361, rok: 2010 ; AIS: 1.238, rok: 2010
DOI: https://doi.org/10.1016/j.bios.2010.08.011

We report on a new assay based on surface plasmon resonance (SPR) which allows for simultaneous 1) real-time monitoring of RNase H activity and 2) detection of cleavage reaction products. The potential of the assay is demonstrated in the study in which the ability of natural and modified oligonucleotides to activate RNase H is examined.
Trvalý link: http://hdl.handle.net/11104/0190575